Interleukin 17 inhibits myogenic and promotes osteogenic differentiation of C2C12 myoblasts by activating ERK1,2

详细信息	查看全文 | 推荐本文 |

• 作者：Jelena Koci膰^a ; ^{jelena.kocic@imi.bg.ac.rs} ; Juan F. Santiba&ntilde ; ez^a ; Aleksandra Krsti膰^a ; Slavko Mojsilovi膰^a ; Ivana Oki膰 膼or膽evi膰^a ; Drenka Trivanovi膰^a ; Vesna Ili膰^b ; Diana Bugarski^a
• 关键词：C2C12 ; Myoblast ; IL-17 ; Myogenic differentiation ; Osteogenic differentiation ; ERK1 ; 2
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Cell Research
• 出版年：2012
• 期刊代码：20_01674889
• 类别：bio
• 出版时间：April, 2012
• 卷：1823
• 期：4
• 页码：838-849
• 文件大小：1197 K

摘要

The present study evaluated the role of interleukin (IL) 17 in multilineage commitment of C2C12 myoblastic cells and investigated associated signaling pathways. The results concerning the effects on cell function showed that IL-17 inhibits the migration of C2C12 cells, while not affecting their proliferation. The data regarding the influence on differentiation demonstrated that IL-17 inhibits myogenic differentiation of C2C12 cells by down-regulating the myogenin mRNA level, myosin heavy chain expression and myotube formation, but promotes their osteogenic differentiation by up-regulating the Runt-related transcription factor 2 mRNA level, cyclooxygenase-2 expression and alkaline phosphatase activity. IL-17 exerted these effects by activating ERK1,2 mitogen activated protein kinase signaling pathway, which in turn regulated the expression of relevant genes and proteins to inhibit myogenic differentiation and induce osteogenic differentiation. Additional analysis showed that the induction of osteogenic differentiation by IL-17 is independent of BMP signaling. The results obtained demonstrate the potential of IL-17 not only to inhibit the myogenic differentiation of C2C12 myoblasts but also to convert their differentiation pathway into that of osteoblast lineage providing new insight into the capacities of IL-17 to modulate the differentiation commitment.