Electrochemical immunoassay of benzo[a]pyrene based on dual amplification strategy of electron-accelerated Fe₃O₄/polyaniline platform and multi-enzyme-functionalized carbon sphere label

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• 作者：Mouhong Lin ; Yingju Liu ; ^{liuyingju@hotmail.com} ; Zihong Sun ; Shenglai Zhang ; Zhuohong Yang ; Chunlin Ni ; ^{niclchem@scau.edu.cn}
• 关键词：Immunosensor ; Benzo[a]pyrene ; Fe3O4/polyaniline ; Carbon nanospheres
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：13 April, 2012
• 卷：722
• 期：Complete
• 页码：100-106
• 文件大小：1039 K

摘要

An electrochemical immunosensor, basing on a dual amplification strategy by employing a biocompatible Fe₃O₄/polyaniline/Nafion (Fe₃O₄/PANI/Nafion) layer as sensor platform and multi-enzyme-antibody functionalized highly-carbonized spheres (multi-HRP-HCS-Ab₂) as label, was constructed for sensitive detection of benzo[a]pyrene (BaP). The stable film, Fe₃O₄/PANI/Nafion, can not only immobilize biomolecules, but also catalyze the reduction of hydrogen peroxide, indicating an accelerated electron transfer pathway of the platform. The experimental conditions, including the concentration of Nafion, concentration of Fe₃O₄/polyaniline (Fe₃O₄/PANI), pH of the detection solution and concentrations of biomolecules, were studied in detail. Basing on a competitive immunoassay, the current change was proportional to the logarithm of BaP concentration in the range of 8 pM and 2 nM with the detection limit of 4 pM. The proposed immunosensor exhibited acceptable reproducibility and stability. This new type of dual amplification strategy may provide potential applications for the detection of environmental pollutants.