Silencing OCILRP2 leads to intrinsic defects in T cells in response to antigenic stimulation
- 作者：Wenzhi Tian ; Biao Feng and Hsiou-Chi Liou
- 关键词：siRNA ; C-type lectin ; T lymphocytes ; Costimulation ; TCR signaling
- 刊名：Cellular Immunology
- 出版年：2005
- 期刊代码：279_00088749
- 类别：med
- 出版时间：2005
- 卷：235
- 期：1
- 页码：72-84
- 文件大小：602 K
摘要
We have previously demonstrated that OCILRP2 interaction with its ligand NKRP1f provides a co-stimulatory signal for optimal T cell proliferation and IL-2 production. Here, using RNA interference technology, we will demonstrate that silencing OCILRP2 in vivo leads to intrinsic impairment in T cell response to CD3- and CD28-cross-linking as well as antigenic stimulation. OCILRP2-silenced T cells have reduced cell proliferation and IL-2 production, which can be bypassed by PMA and ionomycin treatment. OCILRP2-silenced T cells also failed to undergo TCR capping and had impaired cytoskeleton reorganization. Moreover, in OCILRP2-silenced T cells, tyrosine phosphorylation of Lck was diminished, while tyrosine phosphorylation of linkers for activation of T cells was unchanged. Interestingly, NF-κB activation was also impaired as the result of OCILRP2 silencing. Together, our data strongly support a novel role for OCILRP2 C-type lectin in TCR-mediated signal transduction. The observation that OCILRP2 is involved in TCR capping and cytoskeletal organization suggests that OCILRP2-NKRP1f may facilitate lipid rafts and immunological synapse formation during T cell interaction with antigen presenting cells.