Development of monoclonal antibody-based competitive immunoassays for the detection of picoxystrobin in cereal and oilseed flours

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• 作者：Josep V. Mercader^a ; ¹ ; Javier Parra^a ; ¹ ; Francesc A. Esteve-Turrillas^a ; Consuelo Agulló ; ^b ; Antonio Abad-Somovilla^b ; Antonio Abad-Fuentes^a ; ^{aabad@iata.csic.es}
• 关键词：ELISA ; Hapten ; Spacer arm ; Strobilurins ; Pesticide residues ; Food safety
• 刊名：Food Control
• 出版年：2012
• 期刊代码：49_09567135
• 类别：abs
• 出版时间：July, 2012
• 卷：26
• 期：1
• 页码：162-168
• 文件大小：511 K

摘要

Picoxystrobin is a new generation fungicide primarily developed to be used in cereal crops. In the present study a novel collection of specific monoclonal antibodies has been produced using different immunizing haptens based on a carboxy-functionalized identical spacer arm attached to alternative positions of the target pesticide molecule. Two competitive enzyme-linked immunosorbent assays have been developed employing hapten heterology, one using the antibody-coated direct format and the other in the conjugate-coated indirect format. Both immunoassays have been characterized in terms of selectivity, solvent tolerance, and buffer conditions, affording similar limits of detection at or below 0.1聽渭g/L. Finally, the optimized assays were applied to the analysis of picoxystrobin in wheat, corn, oat, barley, and soybean flours. Average recovery values from spiked samples were between 84 and 115%.