Quantitation of protein carbonylation by dot blot

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• 作者：Nancy B. Wehr ; Rodney L. Levine ; ^{rlevine@nih.gov}
• 关键词：Protein carbonylation ; 2 ; 4-Dinitrophenylhydrazine ; Oxidative stress ; Immunoblot ; Dot blot
• 刊名：Analytical Biochemistry
• 出版年：2012
• 期刊代码：93_00032697
• 类别：imm
• 出版时间：15 April, 2012
• 卷：423
• 期：2
• 页码：241-245
• 文件大小：293 K

摘要

Protein carbonylation is the most commonly used measure of oxidative modification of proteins. It is frequently measured spectrophotometrically or immunochemically by derivatizing proteins with the classical carbonyl reagent, 2,4-dinitrophenylhydrazine. We developed an immunochemical dot blot method for quantitation of protein carbonylation in homogenates or purified proteins. Dimethyl sulfoxide was employed as the solvent because it very efficiently extracts proteins from tissues and keeps them soluble. It also readily dissolves 2,4-dinitrophenylhydrazine and wets polyvinylidene difluoride (PVDF) membranes. The detection limit is 0.19 卤 0.04 pmol of carbonyl, and 60 ng of protein is sufficient to measure protein carbonyl content. This level of sensitivity allowed measurement of protein carbonylation in individual Drosophila.