Acute myocardial infarction induces bilateral stellate ganglia neural remodeling in rabbits

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• 作者：Bich Lien Nguyen^a ; ^c ; Hongmei Li^a ; Michael C. Fishbein^b ; Shien-Fong Lin^d ; Carlo Gaudio^c ; Peng-Sheng Chen^a ; ^d ; Lan S. Chen^e ; ^{lschen@iupui.edu}
• 关键词：Myocardial infarction ; Ventricular arrhythmia ; Autonomic nervous system ; Stellate ganglion ; Nerve sprouting ; Sudden cardiac death
• 刊名：Cardiovascular Pathology
• 出版年：2012
• 期刊代码：46_10548807
• 类别：med
• 出版时间：May-June, 2012
• 卷：21
• 期：3
• 页码：143-148
• 文件大小：1185 K

摘要

Introduction

Myocardial infarction (MI) results in cardiac nerve sprouting in the myocardium. Whether or not similar neural remodeling occurs in the stellate ganglia (SGs) is unknown. We aimed to test the hypothesis that MI induces bilateral SG nerve sprouting.

Methods

Acute MI was created by coronary artery ligation in rabbits (n=12). Serum nerve growth factor (NGF) level was measured by enzyme-linked immunosorbent assay. The hearts and bilateral SGs were harvested for immunohistochemistry after 1 week in six rabbits and after 1 month in six rabbits. Immunostaining for tyrosine hydroxylase (TH), growth-associated protein 43 (GAP43), choline acetyltransferase (ChAT), and synaptophysin (SYN) was performed to determine the magnitude of nerve sprouting. Tissues from six normal rabbits were used as controls. Nerve density was determined by computerized morphometry.

Results

Myocardial infarction results in increased serum NGF levels at 1 week (1519.8卤632.2 ng/ml) that persist up to 1 month (1361.2卤176.3 ng/ml) as compared to controls (89.6卤34.9 ng/ml) (P=.0002 and P=.0001, respectively). Immunostaining demonstrated nerve sprouting and hyperinnervation in both SGs after MI. The nerve densities (渭m²/ganglion cell) in SG 1 week after MI and 1 month after MI and those in control groups, respectively, were as follows: GAP43: 278卤96, 225卤39, and 149卤57 (P=.01); SYN: 244卤152, 268卤115, and 102卤60 (P=.02); TH: 233卤71, 180卤50, and 135卤68 (P=.047); ChAT: 244卤100, 208卤46, and 130卤41 渭m²/cell (P=.01).

Conclusions

Myocardial infarction increases serum NGF levels and induces nerve sprouting and hyperinnervation in bilateral SGs for at least 1 month after MI. The hyperinnervation includes both adrenergic axons and cholinergic axons in the SG.