Natural mixtures of persistent organic pollutants (POPs) suppress ovarian follicle development, liver vitellogenin immunostaining and hepatocyte proliferation in female zebrafish (Danio rerio)

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• 作者：Marianne Kraugerud^a ; ^{Marianne.Kraugerud@nvh.no} ; Richard William Doughty^b ; ^{vetrwdoughty@yahoo.co.uk} ; Jan L. Lyche^c ; ^{Jan.Lyche@nvh.no} ; Vidar Berg^c ; ^{Vidar.Berg@nvh.no} ; Nina H. Tremoen^d ; ^{Nina.Hardnes@nvh.no} ; Peter Alestrø ; m^a ; ^{Peter.Alestrom@nvh.no} ; Mona Aleksandersen^a ; ^{Mona.Aleksandersen@nvh.no} ; Erik Ropstad^d ; ^{Erik.Ropstad@nvh.no}
• 关键词：POPs ; Ovary ; Follicle ; Oocyte ; Vitellogenin ; Liver
• 刊名：Aquatic Toxicology
• 出版年：2012
• 期刊代码：19_0166445x
• 类别：abs
• 出版时间：15 July, 2012
• 卷：116-117
• 期：Complete
• 页码：16-23
• 文件大小：1019 K

摘要

Persistent organic pollutants such as polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and dichlorodiphenyltrichloroethane (DDT) are present in high concentrations in livers of burbot (Lota lota) in Lake Mj酶sa, Norway. In order to assess effects of such pollutants on fish gonadal morphology, female zebrafish were exposed in two generations by food to mixtures of pollutants extracted from livers of burbot from Lake Mj酶sa (high and low dose) and Lake Losna, which represents background pollution, and compared to a control group. Ovarian follicle counts detected a significant decrease in late vitellogenic follicle stages in fish exposed to the Losna and the high concentrations of Mj酶sa mixtures in fish from the first generation. In addition, proliferation of granulosa cells, visualized by immunohistochemistry against proliferating cell nuclear antigen (PCNA), was decreased in all exposure groups in either early or late vitellogenic follicle stages compared to control. This was accompanied by increased apoptosis of granulosa cells. There was a decrease in proliferation of liver hepatocytes with exposure to both Mj酶sa mixtures. In addition, immunopositivity for vitellogenin in the liver was significantly lower in the Mj酶sa high group than in the control group. When analysing effects of parental exposure, fish with parents exposed to Mj酶sa high mixture had significantly higher numbers of perinucleolar follicles than fish with control parents.

We conclude that long-term exposure of a real-life mixture of pollutants containing high- and background levels of chemicals supress ovarian follicle development, liver vitellogenin immunostaining intensity and hepatocyte proliferation in the zebrafish model.