Highly efficient magnetic stem cell labeling with citrate-coated superparamagnetic iron oxide nanoparticles for MRI tracking

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• 作者：Kristin Andreas^a ; ^{kristin.andreas@charite.de} ; Radostina Georgieva^b ; Mechthild Ladwig^c ; Susanne Mueller^d ; Michael Notter^e ; Michael Sittinger^a ; Jochen Ringe^a
• 关键词：Mesenchymal stem cell ; Magnetic cell labeling ; Superparamagnetic iron oxide nanoparticles ; Magnetic resonance imaging ; In vivo cell tracking ; Tissue engineering
• 刊名：Biomaterials
• 出版年：2012
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：June, 2012
• 卷：33
• 期：18
• 页码：4515-4525
• 文件大小：2252 K

摘要

Tracking of transplanted stem cells is essential to monitor safety and efficiency of cell-based therapies. Magnetic resonance imaging (MRI) offers a very sensitive, repetitive and non-invasive in聽vivo detection of magnetically labeled cells but labeling with commercial superparamagnetic iron oxide nanoparticles (SPIONs) is still problematic because of low labeling efficiencies and the need of potentially toxic transfection agents. In this study, new experimental citrate-coated SPIONs and commercial Endorem and Resovist SPIONs were investigated comparatively in terms of in聽vitro labeling efficiency, effects on stem cell functionality and in聽vivo MRI visualization. Efficient labeling of human mesenchymal stem cells (MSCs) without transfection agents was only achieved with Citrate SPIONs. Magnetic labeling of human MSCs did not affect cell proliferation, presentation of typical cell surface marker antigens and differentiation into the adipogenic and osteogenic lineages. However, chondrogenic differentiation and chemotaxis were significantly impaired with increasing SPION incorporation. Transplanted SPION-labeled MSCs were visualized in聽vivo after intramuscular injection in rats by 7T-MRI and were retrieved ex聽vivo by Prussian Blue and immunohistochemical stainings. Though a careful titration of SPION incorporation, cellular function and MRI visualization is essential, Citrate SPIONs are very efficient intracellular magnetic labels for in聽vivo stem cell tracking by MRI.