Temperature and density dependent induction of a cytopathic effect following infection with non-cytopathic HAV strains
- 作者：Samantha Q. Wales samantha.wales@fda.hhs.gov ; Diana Ngo diana.ngo@fda.hhs.gov ; Kaoru Hida kaoru.hida@fda.hhs.gov ; Michael Kulka ; michael.kulka@fda.hhs.gov
- 关键词：Hepatitis A ; Cytopathic effect ; Apoptosis ; RNase L
- 刊名：Virology
- 出版年：2012
- 期刊代码：108_00426822
- 类别：imm
- 出版时间：15 August, 2012
- 卷：430
- 期：1
- 页码：30-42
- 文件大小：1411 K
摘要
Hepatitis A virus infection and growth in cultured cells is protracted, cell-type restricted, and generally not accompanied by the appearance of a cytopathic effect, with the exception of some culture-adapted strains. We demonstrate that the non-cytopathic HAV strain HM175/clone 1 can be induced to exhibit a cytopathic phenotype in both persistently or acutely infected cells under co-dependent conditions of lower incubation temperature (<34 掳C) and reduced cell density in both monkey (FRhK-4) and human (A549) cells. This phenotype is not virus-strain restricted, as it was also observed in cells infected with HAV strains, HAS-15 and LSH/S. Cytopathic effect was accompanied by rRNA cleavage, indicating activation of the RNase L pathway, viral negative strand synthesis, caspase-3 activation, and apoptosis. The results indicate that a cytopathic phenotype may be present in some HAV strains that can be induced under appropriate conditions, suggesting the potential for development of a plaque assay for this virus.