- 作者：Kun Liu ; M.D.&lowast ; ; &dagger ; ; Li Shen ; M.D.&lowast ; ; Jun Wang ; M.D.&lowast ; ; Guohua Dong ; M.D.&lowast ; ; Haiwei Wu ; M.D.&lowast ; ; Hongtao Shao ; M.D.&lowast ; ; Hua Jing ; M.D.&lowast ; ; jing_hua_1@163.com
- 关键词：cardiopulmonary bypass ; curcumin ; lung injury ; inflammation response ; rat
- 刊名：Journal of Surgical Research
- 出版年：2012
- 期刊代码：299_00224804
- 类别：med
- 出版时间：1 May, 2012
- 卷：174
- 期：1
- 页码：73-82
- 文件大小：1055 K
Background
Acute lung injury is a frequent complication after cardiopulmonary bypass (CPB). Recent studies have reported that NF-魏B plays an important role in the pathogenesis of post-CPB pulmonary dysfunction. Several signaling pathways, including the TLR4 pathway, induce NF-魏B leading to an inflammatory response. We designed this study to determine whether or not curcumin inhibits TLR4 and MyD88 protein levels and ameliorates lung inflammatory injury in a rat CPB model.Materials and methods
Sprague-Dawley rats were randomly divided into the following five groups (n = 12): sham; control (CPB); vehicle; low-dose curcumin (L-Cur); and high-dose curcumin (H-Cur). The percutaneous beating heart CPB model of rat was established. Animals were pretreated with a single intraperitoneal injection of vehicle, L-Cur (50 mg/kg), or H-Cur (200 mg/kg) 2 h prior to CPB. Blood were sampled at various time points, then lung tissues and bronchoalveolar lavage fluid were harvested 24 h after CPB.
Results
CPB induced a marked increase in the concentrations of interleukin-8, tumor necrosis factor-伪, and matrix metalloproteinase-9 in plasma, bronchoalveolar lavage fluid, and lung tissues (P < 0.05 versus sham group), whereas curcumin pretreatment reduced these inflammatory markers. Curcumin had effective inhibitory effects on the expression of TLR4, MyD88, and NF-魏B in lung tissues 24 h post-CPB (P < 0.05 versus vehicle group). Administration of curcumin remarkably decreased the lung injury score (L-Cur versus vehicle group, P = 0.024; H-Cur versus vehicle group, P = 0.013).
Conclusions
Curcumin may be an alternative therapy for protecting CPB-induced lung injury by suppressing the expression of inflammatory cytokines. This anti-inflammatory effect of curcumin is partly related聽to the inhibition of TLR4, MyD88, and NF-魏B.