High-yield production of a stable Vero cell-based vaccine candidate against the highly pathogenic avian influenza virus H5N1

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• 作者：Fangye Zhou^a ; Jian Zhou^a ; Lei Ma^a ; Shaohui Song^a ; Xinwen Zhang^a ; Weidong Li^a ; Shude Jiang^a ; Yue Wang^b ; ^{euy-tokyo@umin.ac.jp} ; Guoyang Liao^a ; ^{liaogy@21cn.com}
• 关键词：Highly pathogenic avian influenza (HPAI) viruses ; Vero cells ; Yield and stability ; Reverse genetics
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：18 May, 2012
• 卷：421
• 期：4
• 页码：850-854
• 文件大小：258 K

摘要

Highly pathogenic avian influenza (HPAI) viruses pose a global pandemic threat, for which rapid large-scale vaccine production technology is critical for prevention and control. Because chickens are highly susceptible to HPAI viruses, the supply of chicken embryos for vaccine production might be depleted during a virus outbreak. Therefore, developing HPAI virus vaccines using other technologies is critical. Meeting vaccine demand using the Vero cell-based fermentation process has been hindered by low stability and yield. In this study, a Vero cell-based HPAI H5N1 vaccine candidate (H5N1/YNVa) with stable high yield was achieved by reassortment of the Vero-adapted (Va) high growth A/Yunnan/1/2005(H3N2) (YNVa) virus with the A/Anhui/1/2005(H5N1) attenuated influenza vaccine strain (H5N1delta) using the 6/2 method. The reassorted H5N1/YNVa vaccine maintained a high hemagglutination (HA) titer of 1024. Furthermore, H5N1/YNVa displayed low pathogenicity and uniform immunogenicity compared to that of the parent virus.