Use of recombinant modified vaccinia Ankara viral vectors for equine influenza vaccination
- 作者：Breathnach ; C.C. ; Rudersdorf ; R. ; Lunn ; D.P.
- 关键词：MVA ; modified vaccinia Ankara ; HA ; hemagglutinin ; NP ; nucleoprotein ; Eq/Ky ; A/equine/Kentucky/1/81 influenza virus ; AMV ; avian myeloblastosis virus ; BHK-21 ; baby hamster kidney-21 cells ; MMLV ; Moloney’ ; s murine leukemia virus ; Horses ; Influenza virus
- 刊名：Veterinary Immunology and Immunopathology
- 出版年：2004
- 期刊代码：105_01652427
- 类别：abs
- 出版时间：April, 2004
- 卷：98
- 期：3-4
- 页码：127-136
- 文件大小：251 K
摘要
Recombinant modified vaccinia Ankara (MVA) vectors expressing equine influenza virus genes were constructed and evaluated for use in equine vaccination. Two strains of recombinant MVA, expressing either hemagglutinin (HA) or nucleoprotein (NP) genes were constructed. Each influenza virus gene was cloned from A/equine/Kentucky/1/81 (Eq/Ky) into an MVA construction plasmid, and was introduced to the deletion III locus of the wild type MVA genome by homologous recombination. Recombinant viruses were plaque purified, and antigen expression was confirmed by immunostaining.