Evaluation of a vectored equine herpesvirus type 1 (EHV-1) vaccine expressing H3 haemagglutinin in the protection of dogs against canine influenza
- 作者：Cristina Rosas ; Gerlinde R. Van de Walle ; Stephan M. Metzger ; Karin Hoelzer ; Edward J. Dubovi ; Sung G. Kim ; Colin R. Parrish ; Nikolaus Osterrieder
- 关键词：EHV-1 ; Canine influenza ; H3
- 刊名：Vaccine
- 出版年：2008
- 期刊代码：89_0264410x
- 类别：imm
- 出版时间：2 May 2008
- 卷：26
- 期：19
- 页码：2335-2343
- 文件大小：680 K
摘要
In 2004, canine influenza virus (CIV) was identified as a respiratory pathogen of dogs for the first time and found to be closely related to H3N8 equine influenza virus (EIV). We generated a recombinant vectored vaccine that expresses H3 of a recent isolate of EIV using equine herpesvirus type 1 (EHV-1) as the delivery vehicle. This EHV-1 vectored vaccine exhibited robust and stable EIV H3 expression and induced a strong influenza virus-specific response in both mice and dogs upon intranasal or subcutaneous administration. Furthermore, upon challenge with the recent CIV isolate A/canine/PA/10915-07, protection of vaccinated dogs could be demonstrated by a significant reduction in clinical sings, and, more importantly, by a significant reduction in virus shedding. We concluded that the EHV-1/H3 recombinant vector can be a valuable alternative for protection of dogs against clinical disease induced by CIV and can significantly reduce virus spread.