Molecular profiling of bladder cancer: Involvement of the TGF-β pathway in bladder cancer progression
- 作者：Tzong-Tyng Hung ; Hong Wang ; Elizabeth A. Kingsley ; Gail P. Risbridger ; Pamela J. Russell
- 关键词：Bladder cancer ; Bone morphogenetic protein-2 ; Inhibin-β ; B ; Transforming growth factor-beta pathway
- 刊名：Cancer Letters
- 出版年：2008
- 期刊代码：44_03043835
- 类别：med
- 出版时间：28 June 2008
- 卷：265
- 期：1
- 页码：27-38
- 文件大小：3033 K
摘要
A human bladder cancer model of nine cell sublines derived from the BL17/2 cell line was used to evaluate genes related to disease progression. Molecular profiling of sublines that were non-tumorigenic and invasive in nude mice was performed and identified 1367 differentially-expressed genes. Quantitative real-time PCR analysis of six transforming growth factor-beta (TGF-β) pathway genes using the entire panel of nine cell lines was performed. Bone morphogenetic protein-2 expression was significantly associated with in vivo tumorigenicity of the cell lines (p = 0.0228, Mann–Whitney); inhibin-βB was related to their invasiveness (p = 0.0468, Mann–Whitney). Analysis of conditioned medium showed TGF-β1 production to be significantly associated with the phenotype of the cell line. The study shows the possible involvement of the TGF-β pathway in bladder cancer progression.