Knockout of the 纬-aminobutyric acid receptor subunit 伪4 reduces functional 未-containing extrasynaptic receptors in hippocampal pyramidal cells at the onset of puberty

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• 作者：Nicole Sabaliauskas^a ; Hui Shen^b ; Gregg E. Homanics^c ; Sheryl S. Smith^b ; Chiye Aoki^a ; ^{chiye@cns.nyu.edu}
• 关键词：Puberty ; GABA type A receptor ; THP ; Tonic inhibition ; Alpha 4 ; Delta ; Allopregnanolone
• 刊名：Brain Research
• 出版年：2012
• 期刊代码：8_00068993
• 类别：neu
• 出版时间：23 April, 2012
• 卷：1450
• 期：Complete
• 页码：11-23
• 文件大小：1340 K

摘要

Increased plasmalemmal localization of 伪4尾未 GABA_A receptors (GABARs) occurs in the hippocampal pyramidal cells of female mice at pubertal onset (). This increase occurs on both dendritic spines and shafts of CA1 pyramidal cells and is in response to hormone fluctuations that occur at pubertal onset. However, little is known about how the 伪4 and 未 subunits individually mediate the formation of functional, plasmalemmal 伪4尾未 GABARs. To determine whether expression of the 伪4 subunit is necessary for plasmalemmal 未 subunit localization at pubertal onset, electron microscopic-immunocytochemistry (EM-ICC) was employed. CA1 pyramidal cells of female 伪4 knockout (KO) mice were tested for plasmalemmal levels of the 未 subunit within dendritic spine and shaft profiles at the onset of puberty. EM-ICC revealed that the 伪4 and 未 subunits localize on dendritic spines and shafts at sites extrasynaptic to GABAergic input at pubertal onset in tissue of wild-type (WT) mice. At pubertal onset, plasmalemmal localization of the 未 subunit is reduced 45.9%on dendritic spines, and 56.7%on dendritic shafts with KO of the 伪4 subunit, as compared to WT tissue, yet levels of intracellular 未 immunoreactivity remain unchanged. The decline in plasmalemmal localization is manifested as decreased responsiveness to the GABA agonist gaboxadol at concentrations that are selective for 未-containing GABARs. Additionally, 伪4 KO mice have larger dendritic spine and shaft profiles. Our findings demonstrate that 伪4 subunit expression strongly influences the pubertal increase of 未 subunits at the plasma membrane, and that genetic deletion of 伪4 serves as a functional knock-down of 未-containing GABARs.