Standardization of Micro-Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blot for Detection of Trypanosoma cruzi Antibodies Using Extracts from Mexican Strains as Antigens

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• 作者：S&aacute ; nchez ; Beatrí ; z ; Monteó ; n ; Ví ; ctor ; Reyes ; Pedro Antonio ; Espinoza ; Bertha
• 关键词：Trypanosoma cruzi ; Serological diagnosis ; ELISA ; Western blot ; Concordance study
• 刊名：Archives of Medical Research
• 出版年：2001
• 期刊代码：24_01884409
• 类别：med
• 出版时间：September - October, 2001
• 卷：32
• 期：5
• 页码：382-388
• 文件大小：660 K

摘要

This report describes two assays for the detection of anti-Trypanosoma cruzi antibodies using Mexican strains of the parasite and the concordance with two assays previously evaluated at the Instituto Nacional de Cardiología Ignacio Chía showed a Kappa index of 0.96, indicating high concordance between the results obtained at these two laboratories. Finally, ELISA using Ninoa antigen extract was more sensitive than ELISA with an Argentinean extract, which failed to detect individuals in the chronic asymptomatic phase (undetermined phase) of infection.