Integrated biocatalytic process for trehalose production and separation from rice hydrolysate using a bioreactor system

详细信息	查看全文 | 推荐本文 |

• 作者：Shu Wei Chang^a ; Pei Ting Liu^b ; Ling Chih Hsu^b ; Chin Shuh Chen^b ; ^{cschen@mail.nchu.edu.tw} ; Jei Fu Shaw^b ; ^c ; ^d ; ^e ; ^{boplshaw@gate.sinica.edu.tw}
• 关键词：Bio-treatment ; Bioethanol ; Bioreactor ; Enzyme ; Gluconic acid ; Picrophilus torridus ; Rice ; Trehalose
• 刊名：Food Chemistry
• 出版年：2012
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：15 October, 2012
• 卷：134
• 期：4
• 页码：1745-1753
• 文件大小：396 K

摘要

Rice starch can be hydrolyzed into maltose for trehalose bioconversion by enzymatic methods. In this study, we have successfully established an efficient production system for our recombinant PTTS in large scale. Three bio-treatments were developed to simplify the separation and purification of trehalose from complex rice saccharified liquid. The trehalose conversion rate of 64.63 卤 4.05%at 30 掳C can be reached using rice hydrolysate as the substrate in a 5 l fermentor system. By 1%of raw material koji fermentation, the highest concentration of bioethanol (3.61 卤 0.07%) was obtained at 30 掳C for 36 h. After 12 h of reaction time, the gluconic acid (24.47 卤 0.33 mM) was successfully produced by glucose oxidase (40 U/g rice) using residual glucose as a substrate. After the batch/continuous ionic exchange process, the trehalose can be successfully separated, crystallized and identified as 92.6 卤 0.02%purity and 94.2%of the recovery yield, respectively.