Trans-splicing of an artificially split fungal mini-intein
- 作者：Sk ; er Elleuche ; Stefanie Pö ; ggeler
- 关键词：Intein ; Protein splicing ; Penicillium chrysogenum ; PRP8 protein ; Trans-splicing
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：13 April 2007
- 卷：355
- 期：3
- 页码：830-834
- 文件大小：320 K
摘要
Inteins are internal protein domains found inside the coding region of different proteins. They can autocatalytically self-excise from their host protein and ligate the protein flanks, called exteins, with a peptide bond via a post-translational process called protein cis-splicing. In contrast, protein trans-splicing involves inteins split into an N- and a C-terminal domain. Both domains are synthesized as two separate components and each joined to an extein; the intein domains can reassemble and link the joined exteins into one functional protein. In this study, we introduced three split sites into the PRP8 mini-intein of Penicillium chrysogenum and demonstrated for the first time trans-splicing of a fungal PRP8 intein. Two of the sites introduced allowed splicing to occur in trans while the third was not functional.