Sequence requirements for splicing by the Cne PRP8 intein

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• 作者：Esther J. Pearl ; Joel D.A. Tyndall ; Russell T.M. Poulter ; Sigurd M. Wilbanks
• 关键词：Protein splicing ; Cne PRP8 ; Intein ; Cryptococcus
• 刊名：FEBS Letters
• 出版年：2007
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：26 June 2007
• 卷：581
• 期：16
• 页码：3000-3004
• 文件大小：750 K

摘要

The dependence of protein splicing on conserved residues of the Cne PRP8 intein was assessed by alanine scanning mutagenesis in a foreign protein context. Corroboration was obtained for the involvement of residues at the splice junctions and of the conserved threonine and histidine of motif B. Five additional residues were identified as absolutely required for splicing. Variant W151A displayed premature C-terminal cleavage, not seen with other Cne PRP8 mutants. We propose a model whereby W151 acts to prevent premature C-terminal cleavage, favoring complete splicing as opposed to two disjointed cleavage events.