Effect of ovariectomy on contrast agent diffusion into lumbar intervertebral disc: a dynamic contrast-enhanced MRI study in female rats

详细信息	查看全文 | 推荐本文 |

• 作者：Min Deng^a ; James F. Griffith^a ; Xiao-Ming Zhu^a ; Wai S. Poon^b ; Anil T. Ahuja^a ; Yi-Xiang J. Wang^a ; ^{yixiang_wang@cuhk.edu.hk}
• 关键词：Ovariectomy ; Estrogen deficiency ; Lumbar ; Intervertebral disc ; Contrast agent ; Dynamic MRI ; Wash-in
• 刊名：Magnetic Resonance Imaging
• 出版年：2012
• 期刊代码：188_0730725x
• 类别：med
• 出版时间：June, 2012
• 卷：30
• 期：5
• 页码：683-688
• 文件大小：505 K

摘要

Purpose

The purpose was to study the effect of estrogen deficiency on contrast agent diffusion into intervertebral disc in a rat model.

Materials and Methods

Seven-month-old female Sprague-Dawley rats were used. Fourteen rats had ovariectomy, and nine rats had sham surgery. Magnetic resonance imaging (MRI) of sagittal midsection of lumbar spine was performed with a 1.5-T magnet. Dynamic MRI was performed after a bolus injection of Gd-DOTA (0.3 mmol/kg) through tail vein. Eight hundred images were acquired at 0.6 s per acquisition. Regions of interests were drawn over three discs per rat. Maximum enhancement (E_max) and enhancement slope (E_slope) were evaluated. MRI was carried out at baseline and 8 weeks postsurgery.

Result

All disc enhancements demonstrated an initial fast wash-in phase followed by a second slower wash-in phase. For initial wash-in phase, E¹_max and E¹_slope of all rats remained unchanged at the two time points. For second wash-in phase, E²_max and E²_slope of control rats remained unchanged, while with ovariectomized rats, E²_max showed reduction at 8 weeks (4.5%卤5.6%) compared to baseline (10.3%卤6.3%, P=.037), and E²_slope was lower at 8 weeks (0.015卤0.017) than the baseline (0.029卤0.022), although it was not statistically significant (P=.101).

Conclusion

Ovariectomy induced detectable decrease in second wash-in phase of contrast agent into lumbar disc.