Complex formation of CdSe/ZnS/TOPO nanocrystal vs. molecular chaperone in aqueous solution by hydrophobic interaction
- 作者：Hiromi Horiuchi ; Noriya Iwami ; Fumi Tachibana ; Akashi Ohtaki ; Ryo Iizuka ; Tamotsu Zako ; Masaru Oda ; Masafumi Yohda ; Toshiro Tani
- 刊名：Journal of Luminescence
- 出版年：2007
- 期刊代码：54_00222313
- 类别：ch
- 出版时间：November 2007
- 卷：127
- 期：1
- 页码：192-197
- 文件大小：470 K
摘要
Feasibilities to stabilize CdSe/ZnS/trioctylphosphineoxide (TOPO) nanocrystals (quantum dots, QDs) in aqueous solutions with prefoldin macromolecules in their bioactive states are reported. Prefoldin is a jellyfish-shaped hexameric co-chaperone of the group II chaperonins. As a protein folding intermediate is captured within its central cavity, so CdSe/ZnS/TOPO QDs would also be included within this cavity. It is also found the QDs can be much more dispersed in aqueous solutions and suspended for certain period of time by adding trace amount of t-butanol in the buffer prior to the mixing of the QDs mother solution. While biochemical procedures are evaluated with ordinary fluorescence measurements, possible complex formations are also evaluated with TIRFM single-molecule detection techniques.