Genetic diversity and phylogenetic analysis of glycoprotein GP85 of ALV-J isolates from Mainland China between 1999 and 2010: Coexistence of two extremely different subgroups in layers

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• 作者：Wei Pan ^{panwei525@126.com} ; Yulong Gao ^{ylg@hvri.ac.cn} ; Litin Qin ^{qinliting@yahoo.com.cn} ; Wei Ni ^{nikeer59@163.com} ; Zaisi Liu ^{liuzaisi@126.com} ; Binglin Yun ^{yunbingling2010@126.com} ; Yongqiang Wang ^{wyyq1234@163.com} ; Xiaole Qi ^{xiaoleqi@sohu.com} ; Honglei Gao ^{ghl@hvri.ac.cn} ; Xiaomei Wang ; ^{xmw@hvri.ac.cn}
• 关键词：ALV-J ; Layer ; Genetic diversity ; GP85 ; China
• 刊名：Veterinary Microbiology
• 出版年：2012
• 期刊代码：106_03781135
• 类别：abs
• 出版时间：23 April, 2012
• 卷：156
• 期：1-2
• 页码：205-212
• 文件大小：1562 K

摘要

Avian leukosis virus subgroup J (ALV-J), first isolated in 1989, preferentially infects meat-type birds. However, layer flocks in China have experienced outbreaks of this virus since 2008. To understand the genetic diversity of ALV-J in Chinese layers, we compared and analyzed the GP85 gene sequences of 106 ALV-J isolates that were isolated between 1999 and 2010 in Mainland China. The GP85 gene sequences of 41 layer isolates collected from 9 provinces of China between 2008 and 2010 belonged to two separate, highly diverse subgroups and were differentiated from meat-type chicken isolates. When compared to all meat-type isolates from China, Subgroup 1 exclusively contained current layer isolates and seemed to be dominant; all the isolates in this subgroup exhibited gene diversity, and many unique amino acid mutations were present. In contrast, the viruses in Subgroup 2 were perfectly conserved and shared high identity with the prototype meat-type chicken ALV-J strain HPRS-103. The two subgroups contained only two concurrent mutations at the same position. Moreover, most of the isolates in Subgroup 1 had two additional glycosylation sites (at positions 101 and 191) when compared with those in Subgroup 2. Our study provides evidence for the coexistence of two extremely different ALV-J subgroups in Chinese layers from 2008 to 2010, supporting the need for vaccine development and purification measures to prevent ALV-J infection in layers in China.