Functionalization of niobium electrodes for the construction of impedimetric biosensors

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• 作者：S. Helali ; A. Abdelghani ; I. Hafaiedh ; C. Martelet ; M.I. Prodromidis ; T. Albanis ; N. Jaffrezic-Renault
• 关键词：Atrazine ; Niobium electrode ; Anodic niobium oxide ; Electrochemical impedance spectroscopy ; Biosensors
• 刊名：Materials Science and Engineering ; C
• 出版年：2008
• 期刊代码：63_09284931
• 类别：ms
• 出版时间：1 July 2008
• 卷：28
• 期：5-6
• 页码：826-830
• 文件大小：386 K

摘要

This paper describes the development of an impedimetric immunosensor, based on niobium/niobium oxide (Nb/NbOxHy) electrodes, for the detection of atrazine. Niobium oxide was anodically formed onto niobium electrodes at 25 V in 1 M H₂SO₄. Hydrous oxide layers were then silanized with APTES, and using glutaraldehyde as a cross linker, Fab fragment k47 antibody was covalently immobilized onto the surface of the electrodes. Electrochemical impedance spectroscopy (EIS) was used to characterize the building-up of the immunosensors as well as the binding of atrazine to its specific antibody. In presence of ferricyanide redox species and under a cathodic polarization voltage (− 1.2 V versus SCE), the relationship between the concentration of atrazine and the change of the electron transfer resistance value was studied.