Anticoagulant activities of goby muscle protein hydrolysates

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• 作者：Rim Nasri^a ; Ikram Ben Amor^b ; Ali Bougatef^a ; Naima Nedjar-Arroume^c ; Pascal Dhulster^c ; Jalel Gargouri^b ; Maha Karra Châ ; abouni^a ; Moncef Nasri^a ; ^{moncef.nasri@enis.rnu.tn} ; ^{mon_nasri@yahoo.fr}
• 关键词：Goby ; Protein hydrolysates ; Anticoagulant peptides ; Activated partial thromboplastin time ; Thrombin time
• 刊名：Food Chemistry
• 出版年：2012
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：1 August, 2012
• 卷：133
• 期：3
• 页码：835-841
• 文件大小：463 K

摘要

The anticoagulant activities of protein hydrolysates prepared from goby muscle by treatment with various bacterial alkaline proteases were investigated. All proteases exhibited varying degrees of hydrolysis (DH) and all goby protein hydrolysates (GPHs) caused a significant prolongation of both the thrombin time (TT) and the activated partial thromboplastin time (APTT). The hydrolysate generated by the crude protease from Bacillus licheniformis NH1 displayed the highest anticoagulant activity, and the higher TT (about 32 s) at a concentration of 5 mg/mL was obtained with hydrolysate having a DH of 8.86%. This hydrolysate was then fractionated by size exclusion chromatography on a Sephadex G-25 column into five major fractions (F1-F5). Fraction F2, which exhibited the highest anticoagulant activity, was then fractionated by reversed-phase high-performance liquid chromatography. The molecular masses and amino acid sequences of four peptides in peptide sub-fraction F2-6, which exhibited the highest anticoagulant activity, were determined using ESI-MS and ESI-MS/MS, respectively. The structures of these peptides were identified as Leu-Cys-Arg, His-Cys-Phe, Cys-Leu-Cys-Arg and Leu-Cys-Arg-Arg.