Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources

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• 作者：P.L. Ho^a ; ^b ; ^{plho@hkucc.hku.hk} ; W.U Lo^a ; M.K. Yeung^a ; Zhen Li^a ; Jane Chan^a ; K.H. Chow^a ; W.C. Yam^a ; Amy H.Y. Tong^c ; Jessie Y.J. Bao^c ; C.H. Lin^c ; Si Lok^c ; Susan S. Chiu^d
• 关键词：Pets ; Pigs ; Chicken ; Plasmid ; Extended-spectrum beta-lactamases
• 刊名：Veterinary Microbiology
• 出版年：2012
• 期刊代码：106_03781135
• 类别：abs
• 出版时间：6 July, 2012
• 卷：158
• 期：1-2
• 页码：172-179
• 文件大小：445 K

摘要

Few studies have compared CTX-M encoding plasmids identified in different ecological sources. This study aimed to analyze and compare the molecular epidemiology of plasmids encoding CTX-M-14 among strains from humans and animals. The CTX-M-14 encoding plasmids in 160 m>Escherichia colim> isolates from animal faecal (14 pigs, 16 chickens, 12 cats, 8 cattle, 5 dogs and 3 rodents), human faecal (45 adults and 20 children) and human urine (37 adults) sources in 2002-2010 were characterized by molecular methods. The replicon types of the CTX-M-14 encoding plasmids were IncFII (m>nm> = 61), I1-I纬 (m>nm> = 24), other F types (m>nm> = 23), B/O (m>nm> = 10), K (m>nm> = 6), N (m>nm> = 3), A/C (m>nm> = 1), HI1 (m>nm> = 1), HI2 (m>nm> = 1) and nontypeable (m>nm> = 30). The genetic environment, ISm>Ecp1m> - m>blam>_CTX-M-14 - ISm>903m> was found in 89.7%(52/58), 87.7%(57/65) and 86.5%(32/37) of the animal faecal, human faecal and human urine isolates, respectively. Subtyping of the 61 IncFII incompatibility group plasmids by replicon sequence typing, plasmid PCR-restriction fragment length polymorphism and marker genes (m>yacm>, m>malBm>, m>eitA/eitCm> and m>parB/Am>) profiles showed that 31%(18/58), 30.6%(20/65) and 37.8%(14/37) of the plasmids originating from animal faecal, human faecal and human urine isolates, respectively, were pHK01-like. These 52 pHK01-like plasmids originated from diverse human (20 faecal isolates from 2002, 2007 to 2008, 14 urinary isolates from 2004) and animal (all faecal, 1 cattle, 1 chicken, 5 pigs, 9 cats, 1 dog, 1 rodent from 2008 to 2010) sources. In conclusion, this study highlights the importance of the IncFII group, pHK01-like plasmids in the dissemination of CTX-M-14 among isolates from diverse sources.