- 作者：Laura Cristina Jardim Pô ; rtoa ; c ; laurap@dca.ufla.br ; Carlos Henrique de Castrob ; c ; Sí ; lvia Silveira Quintã ; o Savergninic ; Sé ; rgio Henrique Sousa Santosc ; Adaliene Versiani Matos Ferreirac ; d ; Letí ; cia Maria de Souza Cordeiroc ; Deny Bruce de Sousa Sobrinhob ; Robson Augusto Souza Santosc ; Alvair Pinto de Almeidac ; Leida Maria Botionc
- 关键词：Cardiac metabolism ; Isolated perfused heart ; PPAR伪 ; Fatty acid oxidation ; Glycolytic flux ; Lipoprotein lipase
- 刊名：Life Sciences
- 出版年：2012
- 期刊代码：62_00243205
- 类别：imm
- 出版时间：9 April, 2012
- 卷：90
- 期：13-14
- 页码：476-483
- 文件大小：844 K
Aims
As cardiac performance is closely related to its energy supply, our study investigated the effect of the orotic acid cardioprotective agent on the pathways of energy supply, in both conditions of normal flow and ischemia.Main methods
Male Wistar rats were fed during nine days with a balanced diet only or supplemented with 1%orotic acid.
Key findings
Dietary administration of orotic acid increased the cardiac utilization of fatty acids, activity of the lipoprotein lipase, expression of the gene of peroxisome proliferator-activated receptor 伪 and its target enzymes. In addition, orotic acid increased the myocardial uptake and incorporation of glucose, glycogen content and level of GLUT4, concentration of glycolytic metabolites and lactate production in both experimental conditions, baseline and after regional ischemia.
Significance
Thus, in orotic acid hearts there was a simultaneous stimulus of fatty acid oxidation and glycolytic pathway, reflected in increased energetic content even in pre-ischemia. The analysis of the cardiac contractility index showed a positive inotropic effect of orotic acid due, at least in part, to the increased availability of energy. The result allows us to suggest that the metabolic changes induced by orotic acid result in appreciable alterations on myocardial contractile function.