Methylglyoxal-induced stomatal closure accompanied by peroxidase-mediated ROS production in Arabidopsis

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• 作者：Tahsina Sharmin Hoque ; Misugi Uraji ; Wenxiu Ye ; Mohammad Anowar Hossain ; Yoshimasa Nakamura ; Yoshiyuki Murata ; ^{muta@cc.okayama-u.ac.jp}
• 关键词：ABA ; abscisic acid ; [Ca2+]cyt ; cytosolic free calcium concentration ; FDA ; fluorescein diacetate ; H2DCF-DA ; 2虂 ; 7虂-dichlorodihydrofluorescein diacetate ; JA ; jasmonic acid ; MAPK ; mitogen-activated protein kinase ; MeJA ; methyl jasmonate ; MG ; methylglyoxal
• 刊名：Journal of Plant Physiology
• 出版年：2012
• 期刊代码：10_01761617
• 类别：abs
• 出版时间：1 July, 2012
• 卷：169
• 期：10
• 页码：979-986
• 文件大小：642 K

摘要

Methylglyoxal (MG) is an oxygenated short aldehyde and a glycolytic intermediate that accumulates in plants under environmental stresses. Being a reactive 伪-oxoaldehyde, MG may act as a signaling molecule in plants during stresses. We investigated whether MG induces stomatal closure, reactive oxygen species (ROS) production, and cytosolic free calcium concentration ([Ca²⁺]_cyt) to clarify roles of MG in Arabidopsis guard cells. MG induced production of ROS and [Ca²⁺]_cyt oscillations, leading to stomatal closure. The MG-induced stomatal closure and ROS production were completely inhibited by a peroxidase inhibitor, salicylhydroxamic acid (SHAM), but were not affected by an NAD(P)H oxidase mutation, atrbohD atrbohF. Furthermore, the MG-elicited [Ca²⁺]_cyt oscillations were significantly suppressed by SHAM but not by the atrbohD atrbohF mutation. Neither endogenous abscisic acid nor endogenous methyl jasmonate was involved in MG-induced stomatal closure. These results suggest that intrinsic metabolite MG can induce stomatal closure in Arabidopsis accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca²⁺]_cyt oscillations.