Catalases negatively regulate methyl jasmonate signaling in guard cells

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• 作者：Rayhanur Jannat^a ; Misugi Uraji^a ; Mohammad Anowar Hossain^a ; Mohammad Muzahidul Islam^a ; Yoshimasa Nakamura^a ; Izumi C. Mori^b ; ^{imori@rib.okayama-u.ac.jp} ; Yoshiyuki Murata^a
• 关键词：Catalase ; Guard cells ; Hydrogen peroxide ; Methyl jasmonate
• 刊名：Journal of Plant Physiology
• 出版年：2012
• 期刊代码：10_01761617
• 类别：abs
• 出版时间：1 July, 2012
• 卷：169
• 期：10
• 页码：1012-1016
• 文件大小：361 K

摘要

Methyl jasmonate (MeJA)-induced stomatal closure is accompanied by the accumulation of hydrogen peroxide (H₂O₂) in guard cells. In this study, we investigated the roles of catalases (CATs) in MeJA-induced stomatal closure using cat mutants cat2, cat3-1 and cat1 cat3, and the CAT inhibitor, 3-aminotriazole (AT). When assessed with 2鈥?7鈥?dichlorodihydrofluorescein, the reduction of catalase activity by means of mutations and the inhibitor accumulated higher basal levels of H₂O₂ in guard cells whereas they did not affect stomatal aperture in the absence of MeJA. In contrast, the cat mutations and the treatment with AT potentiated MeJA-induced stomatal closure and MeJA-induced H₂O₂ production. These results indicate that CATs negatively regulate H₂O₂ accumulation in guard cells and suggest that inducible H₂O₂ production rather than constitutive elevation modulates stomatal apertures in Arabidopsis.