Characterization of a self-assembled monolayer of thiol on a gold surface and the fabrication of a biosensor chip based on surface plasmon resonance for detecting anti-GAD antibody
- 作者：Lee ; Jung Wook ; Sim ; Sang Jun ; Cho ; Sung Min ; Lee ; Jeewon
- 关键词：Surface plasmon resonance ; Self-assembled monolayer ; Glutamic acid decarboxylase ; Streptavidin ; Immunosensor ; Protein adsorption
- 刊名：Biosensors and Bioelectronics
- 出版年：2005
- 期刊代码：12_09565663
- 类别：ch
- 出版时间：January 15, 2005
- 卷：20
- 期：7
- 页码：1422-1427
- 文件大小：157 K
摘要
A biosensor chip utilizing surface plasmon resonance (SPR) was fabricated for detecting anti-glutamic acid decarboxylase (GAD) antibody, which is an indicator of the presence of type I diabetes mellitus. The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 3-mercaptopropionic acid (3-MPA) to 11-mercaptoundecanoic acid (11-MUA). To determine the surface characteristics of the different alkanethiol monolayers, several quantitative and kinetic measurements were carried out. The extent of immobilization of streptavidin (SA) and biotin-GAD (the anti-GAD receptor) and the immune response of anti-GAD antibody against GAD were measured using the SPR biosensor. The terminal functional group of a thiol has different effects on the adsorption and covalent binding of protein depending on the steric hindrance. The protein chip described herein permits simple, rapid detection of anti-GAD antibody.