Recombinant antibodies containing an engineered B-cell epitope capable of eliciting conformation-specific antibody responses
- 作者：Cook ; Jeremy ; Barber ; Brian H.
- 关键词：Recombinant antibodies ; conformational B-cell epitopes ; influenza hemagglutinin ; protein engineering
- 刊名：Vaccine
- 出版年：1995
- 期刊代码：89_0264410x
- 类别：imm
- 出版时间：1995
- 卷：13
- 期：1
- 页码：1770-1778
- 文件大小：1000 K
摘要
The immunogenicity of a soluble, non-self protein or peptide can be greatly enhanced by injecting this antigen coupled to an antibody specific for class II MHC molecules in the recipient. This adjuvant-independent immunization strategy is known as immunotargeting. We have investigated the ability of a mouse anti-class II MHC antibody to provide the three-dimensional framework for the reconstitution of a heterologous conformational B-cell epitope, specifically the A loop epitope from the influenza virus hemagglutinin (HA). From a panel of three anti-class II MHC immunoglobulin (Ig)-A loop constructs, we found that one of these, an insertion into the FR3 region of the Ig molecule, retained its specificity for mouse I-Ak. Although mouse monoclonal antibodies specific for the A loop region in the HA molecule were unable to react with the Ig-A loop variants, we did find that the heavy chain CDR3 insertion construct was able to elicit an A loop-specific, HA-reactive antibody response when used as an immunogen in rabbits. These results demonstrate the potential for the Ig molecule to function successfully as a structural framework for the reconstitution and presentation of heterologous conformational B-cell epitopes.