Rapid isolation and identification of minor natural products by LC-MS, LC-SPE-NMR and ECD: Isoflavanones, biflavanones and bisdihydrocoumarins from Ormocarpum kirkii

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• 作者：Yong-Jiang Xu^a ; Kenn Foubert^a ; Liene Dhooghe^a ; Filip Lemiè ; re^b ; Sheila Maregesi^c ; ¹ ; Christina M. Coleman^d ; Yike Zou^d ; Daneel Ferreira^d ; Sandra Apers^a ; Luc Pieters^a ; ^{luc.pieters@ua.ac.be}
• 关键词：Ormocarpum kirkii ; Papilionaceae ; LC&ndash ; SPE&ndash ; NMR ; Antiplasmodial ; Isoflavonoids ; Biflavonoids ; Neoflavanoids ; Electronic circular dichroism (ECD)
• 刊名：Phytochemistry
• 出版年：2012
• 期刊代码：85_00319422
• 类别：ch
• 出版时间：July, 2012
• 卷：79
• 期：Complete
• 页码：121-128
• 文件大小：534 K

摘要

The combination of the hyphenated techniques LC-MS and LC-SPE-NMR constitutes a powerful platform for the rapid isolation and identification of minor components from natural sources. Electronic circular dichroism (ECD) is a useful tool to determine the absolute configuration of small quantities of chiral molecules. In order to search for minor constituents present in an Ormocarpum kirkii extract, these techniques were applied for the separation and structure elucidation of a series of isoflavanones, biflavanones and biscoumarins. After optimization of chromatographic conditions and subsequent isolation, MS and 1D and 2D NMR data were collected. Experimental and calculated ECD spectra were used in conjunction with NMR data to confirm the absolute configuration of these compounds. Eight compounds were identified for the first time and six have been previously reported. The present approach offers a strategy for accelerating research on natural products.