miR-181b Promotes hepatic stellate cells proliferation by targeting p27 and is elevated in the serum of cirrhosis patients

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• 作者：Baocan Wang^a ; ¹ ; Wenxi Li^b ; ¹ ; Kun Guo^c ; ¹ ; Yongtao Xiao^d ; Yuqin Wang^a ; ^{wangvuqin00@sina.com} ; Jiangao Fan^a ; ^{fanjiangao@gmail.com}
• 关键词：HSC ; hepatic stellate cells ; miRNA ; microRNA ; 3&prime ; -UTR ; 3&prime ; -untranslated region ; TGF-尾1 ; transforming growth factor-beta 1 ; DMEM ; Dulbecco&rsquo ; s modified Eagle&rsquo ; s medium ; FBS ; fetal bovine serum ; 伪-SMA ; alpha smooth muscle actin
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：27 April, 2012
• 卷：421
• 期：1
• 页码：4-8
• 文件大小：549 K

摘要

MicroRNAs, as a kind of negative gene regulators, were demonstrated to be involved in many types of diseases. In this study, we found that transforming growth factor-beta 1 could induce the expression of miR-181a and miR-181b, and miR-181b increased in the much higher folds than miR-181a. Because of the important role of transforming growth factor-beta 1 in HSC activation and liver cirrhosis, we investigate the effect of miR-181a and miR-181b on HSC proliferation. The results showed that miR-181b could promote HSC-T6 cell proliferation by regulating cell cycle. Further study showed p27, the cell cycle regulator, was the direct target of miR-181b in HSC-T6 cell. But miR-181a had no effects on HSC-T6 cell proliferation and cell cycle, and did not target p27. Interestingly, miR-181b is elevated significantly in serum of liver cirrhosis cases comparing to that of normal persons, whereas miR-181a expression was in the similar level with that of normal persons. These results suggested that miR-181b could be induced by TGF-尾1 and promote the growth of HSCs by directly targeting p27. The elevation of miR-181b in serum suggested that it may be potential diagnostic biomarkers for cirrhosis. As for miR-181a, it may work in TGF-尾1 pathway by a currently unknown mechanism.