Human Dectin-1 isoform E is a cytoplasmic protein and interacts with RanBPM
- 作者：Jianhui Xie ; Maoyun Sun ; Liang Guo ; Weicheng Liu ; Jianhai Jiang ; Xiaoning Chen ; Lei Zhou and Jianxin Gu
- 关键词：hDectin-1E ; RanBPM ; C-type lectin-like domain ; Yeast two-hybrid
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2006
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：8 September 2006
- 卷：347
- 期：4
- 页码：1067-1073
- 文件大小：1418 K
摘要
Human Dectin-1, a type II transmembrane receptor, is alternatively spliced, generating eight isoforms. Of these isoforms, the isoform E (hDectin-1E) is structurally unique, containing a complete C-type lectin-like domain as well as an ITAM-like sequence. So far, little is known about its function. In the present study, we demonstrated that hDectin-1E was not secreted and it mainly resided in the cytoplasm. Using yeast two-hybrid screening, we identified a Ran-binding protein, RanBPM, as an interacting partner of hDectin-1E. GST pull-down assays showed that RanBPM interacted directly with hDectin-1E and the region containing SPRY domain was sufficient for the interaction. The binding of hDectin-1E and RanBPM was further confirmed in vivo by co-immunoprecipitation assay and confocal microscopic analysis. Taken together, our data provide a clue to the understanding of the function about hDectin-1E.