The cDNA cloning and mRNA expression of cytoplasmic Cu, Zn superoxide dismutase (SOD) gene in scallop Chlamys farreri
- 作者：Duojiao Ni ; Linsheng Song ; Qiang Gao ; Longtao Wu ; Yundong Yu ; Jianmin Zhao ; Limei Qiu ; Huan Zhang ; Fangfang Shi
- 关键词：Scallop ; Chlamys farreri ; Reactive oxygen species (ROS) ; Superoxide dismutase (SOD) ; Innate immunity ; mRNA expression
- 刊名：Fish & Shellfish Immunology
- 出版年：2007
- 期刊代码：115_10504648
- 类别：abs
- 出版时间：November 2007
- 卷：23
- 期：5
- 页码：1032-1042
- 文件大小：1259 K
摘要
Cu, Zn superoxide dismutases (SODs) are metalloenzymes that represent one important line of defence against reactive oxygen species (ROS). A cytoplasmic Cu, Zn SOD cDNA sequence was cloned from scallop Chlamys farreri by the homology-based cloning technique. The full-length cDNA of scallop cytoplasmic Cu, Zn SOD (designated CfSOD) was 1022 bp with a 459 bp open reading frame encoding a polypeptide of 153 amino acids. The predicted amino acid sequence of CfSOD shared high identity with cytoplasmic Cu, Zn SOD in molluscs, insects, mammals and other animals, such as cytoplasmic Cu, Zn SOD in oyster Crassostrea gigas (CAD42722), mosquito Aedes aegypti (ABF18094), and cow Bos taurus (XP_584414). A quantitative reverse transcriptase real-time PCR (qRT-PCR) assay was developed to assess the mRNA expression of CfSOD in different tissues and the temporal expression of CfSOD in scallop challenged with Listonella anguillarum, Micrococcus luteus and Candida lipolytica respectively. Higher-level mRNA expression of CfSOD was detected in the tissues of haemocytes, gill filaments and kidney. The expression of CfSOD dropped in the first 8–16 h and then recovered after challenge with L. anguillarum and M. luteus, but no change was induced by the C. lipolytica challenge. The results indicated that CfSOD was a constitutive and inducible acute-phase protein, and could play an important role in the immune responses against L. anguillarum and M. luteus infection.