Post-transcriptional CD59 gene silencing by siRNAs induces enhanced human T lymphocyte response to tumor cell lysate-loaded DCs

详细信息	查看全文 | 推荐本文 |

• 作者：Xi-He Xie^a ; ^b ; ^c ; Mei-Hua Gao^a ; ^{mhgao2010@163.com} ; Bei Zhang^a ; Mei-Juan Wang^a ; Juan Wang^a
• 关键词：CD59 ; Human ; T cell activation ; Dendritic cells ; RNA interference ; Regulatory T cells
• 刊名：Cellular Immunology
• 出版年：2012
• 期刊代码：279_00088749
• 类别：med
• 出版时间：2012
• 卷：274
• 期：1-2
• 页码：1-11
• 文件大小：1621 K

摘要

CD59 is a complement regulatory protein known to prevent the membrane attack complex (MAC) from assembling. To investigate the role of CD59 molecules in human T cell activation in response to exogenous antigens, gene silencing via small interfering RNAs (siRNAs) was carried out. Subsequent T cell activation in response to both autologous dendritic cells (DCs) loaded with tumor lysate and beads coated with anti-CD3, anti-CD28 and anti-CD59 antibodies was investigated. The findings demonstrated that decreased CD59 expression on T cells significantly enhanced activation and proliferation of CD4⁺ T cells and CD8⁺ T cells while the expansion of CD4⁺ CD25⁺ regulatory T cells (Tregs) was not affected, and CD59 mediated inhibition of T cell activation requires the binding of CD59 with its ligand on antigen-presenting cells (APCs). The data support that CD59 down-regulates antigen-specific activation of human T lymphocytes in a ligand-dependent manner.