Characterisation of the immunoglobulin variable region gene usage encoding the murine anti-ganglioside antibody repertoire
- 作者：Judith Boffey ; Masaaki Odaka ; Dawn Nicoll ; Eric R. Wagner ; Kate Townson ; Tyrone Bowes ; Joe Conner ; Koichi Furukawa and Hugh J. Willison
- 关键词：GD3 synthase ; Ganglioside ; Neuropathy ; Monoclonal antibody ; Variable region gene
- 刊名：Journal of Neuroimmunology
- 出版年：2005
- 期刊代码：32_01655728
- 类别：neu
- 出版时间：2005
- 卷：165
- 期：1-2
- 页码：92-103
- 文件大小：531 K
摘要
Neuropathogenic murine antibodies reactive with terminal disialylgalactose epitopes are innate and preferentially encoded by the VH7183.3b gene. Here we have studied antibodies reactive with internal galactose-linked disialosyl epitopes and the terminal trisaccharide of GT1b. Antibodies were of moderate affinity and unmutated. Anti-GD1b antibodies were often encoded by the VH10.2b heavy and gj38c light chain genes. Anti-GT1b antibodies with broader glycan binding patterns were encoded by VHQ52 and VHJ558 family genes. These data indicate that the discrete specificities of ganglioside-binding antibodies are dictated by particular patterns of V gene usage residing within the innate B cell repertoire.