Iron-mediated free-radical formation of signaling lipids in a model system

详细信息	查看全文 | 推荐本文 |

• 作者：Irina Yurkova ; Mikhail Kisel ; Juergen Arnhold and Oleg Shadyro
• 关键词：Cardiolipin ; Galactosylceramide ; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ; Fragmentation ; Iron ; Free-radicals ; Phosphatidic acid ; Ceramide
• 刊名：Chemistry and Physics of Lipids
• 出版年：2005
• 期刊代码：44_00093084
• 类别：bio
• 出版时间：2005
• 卷：137
• 期：1-2
• 页码：29-37
• 文件大小：206 K

摘要

It has been shown using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and its combination with thin-layer chromatography (TLC) that the action of the ascorbate/Fe²⁺/H₂O₂ oxidizing system on cardiolipin and galactocerebroside results in the formation of phosphatidic acid (PA) and ceramide (Cer), respectively. These data, when combined with results obtained on radiolysis of similar substances, allowed the conclusion that the formation of PA and Cer occurs via an OH-induced fragmentation taking place in polar moiety of the starting substrates.