Effects of catechin on homocysteine metabolism in hyperhomocysteinemic mice
- 作者：Julien Hamelet ; Karine Demuth ; Julien Dairou ; Auré ; lie Ledru ; Jean-Louis Paul ; Jean-Marie Dupret ; Jean-Maurice Delabar ; Fern ; o Rodrigues-Lima ; Nathalie Janel
- 关键词：Homocysteine ; Cystathionine beta synthase ; Mice ; Liver ; Polyphenols ; Catechin ; Quercetin
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：30 March 2007
- 卷：355
- 期：1
- 页码：221-227
- 文件大小：273 K
摘要
We have recently focused on the interaction between hyperhomocysteinemia, defined by high plasma homocysteine levels, and paraoxonase-1 expression and found a reduced activity of paraoxonase-1 associated with a reduced gene expression in the liver of cystathionine beta synthase (CBS) deficient mice, a murine model of hyperhomocysteinemia. As it has been demonstrated that polyphenolic compounds could modulate the expression level of the paraoxonase-1 gene in vitro, we have investigated the possible effect of flavonoid supplementation on the impaired paraoxonase-1 gene expression and activity induced by hyperhomocysteinemia and have evaluated the link with homocysteine metabolism. High-methionine diet significantly increased serum homocysteine levels, decreased hepatic CBS activity, and down-regulated paraoxonase-1 mRNA and its activity. However, chronic administration of catechin but not quercetin significantly reduced plasma homocysteine levels, attenuated the reduction of the hepatic CBS activity, and restored the decreased paraoxonase-1 gene expression and activity induced by chronic hyperhomocysteinemia. These data suggest that catechin could act on the homocysteine levels by increasing the rate of catabolism of homocysteine.