Probiotic suppression of the H. pylori-induced responses by conjugated linoleic acids in a gastric epithelial cell line

详细信息	查看全文 | 推荐本文 |

• 作者：Sung Wook Hwang^a ; Nayoung Kim^a ; ^b ; ^{nayoungkim49@empal.com} ; Jung Mogg Kim^c ; Chul Sung Huh^d ; Young Tae Ahn^d ; Se Hoon Park^d ; Cheol Min Shin^b ; Ji Hyun Park^a ; Mi Kyoung Lee^a ; Ryoung Hee Nam^b ; Hye Seung Lee^e ; Joo Sung Kim^a ; Hyun Chae Jung^a ; In Sung Song^a
• 关键词：Helicobacter pylori ; Probiotics ; Conjugated linoleic acids ; Interleukin-8 ; Tumor necrosis factor-alpha ; Inducible nitric oxide synthase.
• 刊名：Prostaglandins ; Leukotrienes and Essential Fatty Acids (PLEFA)
• 出版年：2012
• 期刊代码：32_09523278
• 类别：bio
• 出版时间：June, 2012
• 卷：86
• 期：6
• 页码：225-231
• 文件大小：416 K

摘要

Conjugated linoleic acids (CLA) produced by Lactobacillus acidophilus was reported to decrease the activation of nuclear factor-kappa B. CLA was suggested as one of the anti-inflammatory molecular mechanisms of probiotics. In the present study, the effects of CLA on H. pylori-induced multiple responses were evaluated. IL-8, TNF-伪 and iNOS were measured in mRNA and/or protein levels in AGS cells after pretreatment with CLA or CLA-containing conditioned medium (CM) produced by Lactobacillus acidophilus or Lactobacillus plantarum. The increased expressions of IL-8 mRNA/protein and TNF-伪 mRNA were significantly suppressed by pretreatment with CM or CLA. The levels of IL-8 protein and TNF-伪 mRNA were suppressed by CM pretreatment better than CLA. The expression of iNOS mRNA was also significantly inhibited by CM pretreatment. These results suggest that the suppression of multiple mediators by CLA-containing CM plays a key role in the anti-inflammatory and anti-carcinogenic effects of probiotics on H. pylori infection.