Identification of five mouse μ-opioid receptor (MOR) gene (Oprm1) splice variants containing a newly identified alternatively spliced exon

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• 作者：Glenn A. Doyle ; X. Rebecca Sheng ; Sharon S.J. Lin ; Danielle M. Press ; Dorothy E. Grice ; Russell J. Buono ; Thomas N. Ferraro ; Wade H. Berrettini
• 关键词：Murine ; Opiate receptor ; C57BL/6 ; DBA/2 ; cDNA
• 刊名：Gene
• 出版年：2007
• 期刊代码：73_03781119
• 类别：bio
• 出版时间：15 June 2007
• 卷：395
• 期：1-2
• 页码：98-107
• 文件大小：1228 K

摘要

The mouse μ-opioid receptor gene, <i>Oprm1i>, currently contains 18 recognized alternatively spliced exons [Doyle, G.A., Sheng, X.R., Lin, S.S.J., Press, D.M., Grice, D.E., Buono, R.J., Ferraro, T.N., Berrettini, W.H., 2007. Identification of three mouse μ-opioid receptor (MOR) gene (<i>Oprm1i>) splice variants containing a newly identified alternatively spliced exon. Gene 388 (1–2) 135–147, in press (doi:10.1016/j.gene.2006.10.017). Electronic publication 2006 November 1] that generate 27 splice variants encoding at least 11 morphine-binding isoforms of the receptor. Here, we identify five MOR variants that contain an as yet undescribed exon (exon 19) of the gene, and we provide evidence that these MOR splice variants are expressed in the C57BL/6 and DBA/2 mouse strains. Three splice variants, MOR-1Eii, MOR-1Eiii and MOR-1Eiv, encode the MOR-1E isoform and contain the newly identified exon 19 in their 3′ untranslated regions. The fourth splice variant encodes a novel μ-opioid receptor isoform, MOR-1U, and contains exon 19 in its coding region. The cytoplasmic tail of the putative MOR-1U isoform contains a putative nuclear localization signal encoded by the sequence of exon 19. Exon 19 appears to be conserved in the rat, but not in humans. In mouse and rat <i>Oprm1i>, exon 19 is located between described exons 7 and 8. We also report the cloning of the “full-length” MOR-1T splice variant [Kvam, T.-M., Baar, C., Rakvag, T.T., Kaasa, S., Krokan, H.E., Skorpen, F., 2004. Genetic analysis of the murine μ-opioid receptor: increased complexity of <i>Oprm1i> gene splicing, J. Mol. Med. 82 (4) 250–255] that encodes MOR-1 and contains the newly identified exon in its 3′ UTR. RT-PCR analysis suggests that splice variants MOR-1Eii, MOR-1Eiii, MOR-1Eiv, MOR-1T and MOR-1U are expressed in all brain regions analyzed (cortex, cerebellum, hypothalamus, thalamus and striatum). These exon 19-containing splice variants add to the growing complexity of the mouse <i>Oprm1i> gene.