Different pathways of the release of cytotoxic proteins in LAK cells

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• 作者：Sashchenko ; L.P. ; Lukyanova ; T.I. ; Kabanova ; O.D. ; Mirkina ; I. ; Yatskin ; O.N> ; Pongor ; S. ; et. al.
• 关键词：Lymphokine-activated killer cells ; secretion ; Calcium ions ; Cytotoxic proteins
• 刊名：Immunology Letters
• 出版年：1996
• 期刊代码：115_01652478
• 类别：med
• 出版时间：October, 1996
• 卷：53
• 期：1
• 页码：25-29
• 文件大小：368 K

摘要

Human LAK cells were shown to release cytotoxic proteins by both Ca²⁺-dependent and Ca²⁺-independent mechanisms. CD3⁺ CD8⁺ CD16⁻ and CD16⁺ CD8⁺ CD3⁻ LAK cells were co-incubated with target cells in the presence of 4 mM EGTA. Although EGTA inhibited the exocytosis of cytolytic granules, supernatants obtained were cytotoxic for target cells. Cytotoxicity of CD3⁺ LAK cells and CD16⁺ LAK cells was due to cytotoxic proteins with MW 75 (p75), 35 (p35) and 22 (p22) kDa. LAK cells were also shown to release cytotoxic proteins by way of continuous secretion. After co-incubation in the absence of target cells LAK cells can secrete cytotoxic proteins with MW 75 (p75), 55 (p55), 38 (p38), 35 (p35), 25 (p25), 22 (p22) and 17 (p17) kDa.