Novel Rath peptide for intracellular delivery of protein and nucleic acids
- 作者：Manish V. Bais ; Satish Kumar ; Ashok K. Tiwari ; Ranjit S. Kataria ; Viswas K. Nagaleekar ; Sameer Shrivastava ; Kantaraja Chindera
- 关键词：CPP ; Rath peptide ; Peptide based delivery ; Delivery vehicle ; Carrier peptide
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2008
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：23 May 2008
- 卷：370
- 期：1
- 页码：27-32
- 文件大小：2733 K
摘要
In the present study, a novel cell penetrating peptide (CPP) named as Rath, has been identified from the avian infectious bursal disease virus. It has the potential to penetrate and translocate cargo molecules into cells independent of temperature. Additionally, it can deliver oligonucleotide in 30 min and antibodies within an hour intracellular to chicken embryonic fibroblast primary cells. As an ideal delivery vehicle, it has the ability to protect the cargo molecules in the presence of serum, nucleases and has minimal or no cytotoxicity at even higher peptide concentrations studied. The biophysical characterizations showed that Rath has a dominant β structure with a small ![]()
src="http://www.sciencedirect.com/scidirimg/entities/204e.gif" alt="greek small letter alpha" title="greek small letter alpha" border="0"> helix and has remarkable binding ability with protein and DNA. Thus, the characterization of unique Rath peptide to deliver protein or nucleic acid into the cells with non-covalent interaction could be used as an effective delivery method for various cell based assays.