Static and perfusion culture of adult neocortex
摘要
When 400 microns-thick adult neocortical slices were incubated in a commercially-available CO2 incubator with D-MEM/F-12, field potentials evoked by underlying white matter stimulation disappeared within 4 hours. On the other hand, a recording over long hours was possible by continuous culture medium perfusion. We found that several hours were needed until humidity and gas composition in the dish reached an equilibrium with the atmosphere inside the incubator by the previous method. By improving the incubating system so that adequate humidity and pH was obtained, field responses was obtained even in static medium.