Mediatophore regulates acetylcholine release from T cells

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• 作者：Takeshi Fujii^a ; ^{tfujii@dwc.doshisha.ac.jp} ; Yuki Takada-Takatori^a ; Kazuhide Horiguchi^b ; Koichiro Kawashima^c
• 关键词：Acetylcholine ; Choline acetyltransferase ; Mediatophore ; Organic cation transporter ; Vacuolar H+-ATPase (V-ATPase)
• 刊名：Journal of Neuroimmunology
• 出版年：2012
• 期刊代码：32_01655728
• 类别：neu
• 出版时间：March, 2012
• 卷：244
• 期：1-2
• 页码：16-22
• 文件大小：689 K

摘要

Immunological stimulation of T cells by phytohemagglutinin (PHA) enhances the synthesis and release of acetylcholine (ACh), suggesting a role for the lymphocytic cholinergic system in the regulation of immune function. In the present study, we used two human leukemic T cell lines as models to investigate whether mediatophore, a homooligomer of a 16-kDa subunit homologous to the proteolipid subunit c of vacuolar H⁺-ATPase (V-ATPase), is involved in mediating ACh release from T cells. Immunohistochemical analysis revealed the presence of mediatophore in the cytoplasm and on the plasma membrane of both T cell lines. Mediatophore gene expression was up-regulated by immunological T cell activation by PHA. Transfection of anti-mediatophore small interference RNA down-regulated mediatophore gene expression and significantly reduced ACh release. These results suggest that T cells express mediatophore, which then plays a key role in mediating ACh release, and that mediatophore expression is regulated by immunological stimulation.