NO detection in biological samples: Differentiation of ¹⁴NO and ¹⁵NO using infrared laser spectroscopy

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• 作者：Thomas Fritsch ; Paris Brouzos ; Kathrin Heinrich ; Malte Kelm ; Tienush Rassaf ; Peter Hering ; Petra Kleinbongard ; Manfred Mü ; rtz
• 关键词：¹⁵NO ; Laser spectroscopy ; Triiodide chemiluminescence ; Cavity leak-out spectroscopy
• 刊名：Nitric Oxide
• 出版年：2008
• 期刊代码：181_10898603
• 类别：bio
• 出版时间：August 2008
• 卷：19
• 期：1
• 页码：50-56
• 文件大小：727 K

摘要

Accurate characterization of the biochemical pathways of nitric oxide (NO) is essential for investigations in the field of NO research. To analyze the different reaction pathways of enzymatic and non-enzymatic NO formation, determination of the source of NO is crucial. Measuring NO-related products in biological samples distinguishing between ¹⁴NO and ¹⁵NO offers the opportunity to specifically analyze NO signaling in blood and tissue. The aim of this study was to establish a highly sensitive technique for the specific measurement of NO in an isotopologue-selective manner in biological samples.