Kinetic study of biological hydrogen production by anaerobic fermentation

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• 作者：Wen-Hsing Chen ; Shen-Yi Chen ; Samir Kumar Khanal ; Shihwu Sung
• 关键词：Anaerobic fermentation ; Food waste ; Carbohydrate ; Hydrogen production ; Michaelis– ; Menten
• 刊名：International Journal of Hydrogen Energy
• 出版年：2006
• 期刊代码：42_03603199
• 类别：ch
• 出版时间：December 2006
• 卷：31
• 期：15
• 页码：2170-2178
• 文件大小：300 K

摘要

The growth kinetics of hydrogen producing bacteria using three different substrates, namely sucrose, non-fat dry milk (NFDM), and food waste were investigated in dark fermentation through a series of batch experiments. The results showed that hydrogen production potential and hydrogen production rate increased with an increasing substrate concentration. The maximum hydrogen yields from sucrose, NFDM, and food waste were 234, 119, and 101 mL/g COD, respectively. The low pH (pH<4) inhibited hydrogen production and resulted in lower carbohydrate fermentation at high substrate concentration. Michaelis–Menten equation was employed to model the hydrogen production rate at different substrate concentrations. The equation gave a good approximation of the maximum hydrogen production rate and the half saturation constant (K_s) with correlation coefficient (R²) over 0.85. The K_s values of sucrose, NFDM, and food waste were 1.4, 6.6, and 8.7 g COD/L, respectively. Based on K_s values, the substrate affinity of the enriched hydrogen producing culture was found to depend on carbohydrate content of the substrate. The substrate containing high carbohydrate showed a lower K_s value. The maximum hydrogen production rate was governed by the complexity of carbohydrates in the substrate.