Influence of arginine-glycine-aspartic acid (RGD), integrins (α_V and α₅) and osteopontin on bovine sperm–egg binding, and fertilization in vitro

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• 作者：R.F. Gonç ; alves ; C.D. Wolinetz ; G.J. Killian
• 关键词：Integrin ; Osteopontin ; Secreted phosphatase 1 ; Sperm– ; egg binding ; Fertilization ; Bovine
• 刊名：Theriogenology
• 出版年：2007
• 期刊代码：98_0093691x
• 类别：abs
• 出版时间：February 2007
• 卷：67
• 期：3
• 页码：468-474
• 文件大小：199 K

摘要

Osteopontin (OPN), a phosphoprotein containing an arginine-glycine-aspartic acid (RGD) sequence, has been identified in cow oviduct epithelium and fluid. To investigate the potential role OPN in fertilization, we evaluated the ability of RGD peptide (arginine-glycine-aspartic), RGE peptide (arginine-glycine-glutamic acid), integrins α_V and α₅ antibodies and OPN antibody to influence bovine in vitro sperm–egg binding and fertilization. Treatment of sperm or oocytes with the RGD peptide prior fertilization significantly decreased in vitro sperm–egg binding and fertilization compared to the non-treated controls or those treated with RGE peptide. Binding and fertilization were also significantly decreased when in vitro matured bovine oocytes or sperm were pre-incubated with integrins α_V and α₅ antibodies at concentration ranging from 5 to 20 μg/mL. Addition of a rabbit polyclonal IgG antibody against purified bovine milk OPN with sperm or/and oocytes decreased (P < 0.05) fertilization compared to the in vitro-fertilized control. These data provided evidence that integrin ligands existed on bovine oocytes and spermatozoa that contained RGD recognition sequences, and that antibody to OPN, a protein that contains that RGD sequence, was capable of reducing sperm–egg binding and fertilization in vitro.