Distribution of alkane-degrading bacterial communities in soils from King George Island, Maritime Antarctic

详细信息	查看全文 | 推荐本文 |

• 作者：Diogo Jurelevicius^a ; Simone Raposo Cotta^a ; Raquel Peixoto^b ; Alexandre Soares Rosado^b ; Lucy Seldin^a ; ^{lseldin@micro.ufrj.br} ; ^{lucy@seldin.com.br}
• 关键词：Maritime Antarctic ; Alkane-degrading bacteria ; Alkane monooxygenase ; Bioremediation
• 刊名：European Journal of Soil Biology
• 出版年：2012
• 期刊代码：43_11645563
• 类别：abs
• 出版时间：July-August, 2012
• 卷：51
• 期：Complete
• 页码：37-44
• 文件大小：450 K

摘要

The structure of alkane-degrading bacterial communities, which are present in both the hydrocarbon-polluted and pristine soils of King George Island in Maritime Antarctic, was studied using molecular methods. Polymerase Chain Reaction (PCR) amplifications of the alkane monooxygenase AlkB-coding genes, followed by Restriction Fragment Length Polymorphism (PCR-RFLP) and Denaturing Gradient Gel Electrophoresis (PCR-DGGE) analyses, revealed the widespread presence and complex diversity of alkane-utilizing bacteria in these soils. The resulting dendrograms and Canonical Correspondence Analyses (CCA) of PCR-RFLP and PCR-DGGE patterns showed that the characteristics of the different soils, such as physicochemical properties, soil type and/or hydrocarbon contamination levels, affect the distribution of alkane-degrading bacteria. Sequencing of 20 DGGE bands revealed the presence in Antarctic soils of alkane monooxygenases with low similarity (61-91%) compared to those previously described in Gram-positive bacteria, such as Mycobacterium, Gordonia, Rhodococcus and Aeromicrobium. The high diversity of alkB genes in the soils of King George Island suggests the potential for oil pollutant degradation.