Microinjection of the preferential dopamine receptor D3 agonist 7-hydroxy-N,N-di-n-propylaminotetralin hydrobromide into the hypothalamic medial preoptic area induced ejaculation in anesthe
- 作者：N.D. Kitrey ; P. Clé ; ment ; J. Bernabé ; L. Alex ; re ; F. Giuliano
- 关键词：sexual physiology ; brain ; pharmacology ; bulbospongiosus muscles ; seminal vesicle pressure
- 刊名：Neuroscience
- 出版年：2007
- 期刊代码：50_03064522
- 类别：neu
- 出版时间：9 November 2007
- 卷：149
- 期：3
- 页码：636-641
- 文件大小：801 K
摘要
The pivotal role of the medial preoptic area (MPOA) of the hypothalamus in the dopaminergic cerebral control of ejaculation has been investigated for years; nevertheless the function of different dopamine receptors subclasses and their exact interrelations merit additional research.<p>One hundred nanograms of a preferential D3 agonist 7-OH-DPAT (7-hydroxy-N,N-di-n-propylaminotetralin hydrobromide) was microinjected unilaterally into the MPOA of male rats anesthetized with urethane. An ejaculation-related response (bulbospongiosus muscles rhythmic contractions and/or seminal vesicle pressure increases and/or expulsion of a semen plug) was observed in 8 of 10 rats devoid of sexual stimuli, while a similar response was observed in only one rat administered with 10 ng of 7-OH-DPAT. The effect of 7-OH-DPAT 100 ng was mostly abolished by simultaneous MPOA microinjection of 300 ng of a preferential D3 antagonist N-[(n-butyl-2-pyrrolidinyl) methyl]-1-methoxy-4-cyanonaphthalene-2-carboxamide tartrate (nafadotride).<p>Our results support the hypothesis that supraspinal command of ejaculation is mediated by D2-like receptors, probably by D3 receptors, in the MPOA, and draw attention to the idea of these receptors serving as a promising target for pharmacological treatment of human ejaculatory disorders.