Outer membrane protein 25-a mitochondrial anchor and inhibitor of stress-activated protein kinase-3
- 作者：Court ; Naomi W. ; Ingley ; Evan ; Klinken ; S. Peter ; Bogoyevitch ; Marie A.
- 关键词：Stress-activated protein kinase-3 ; OMP25 ; Mitochondria ; PDZ domain
- 刊名：BBA - Molecular Cell Research
- 出版年：2005
- 期刊代码：20_01674889
- 类别：bio
- 出版时间：May 15, 2005
- 卷：1744
- 期：1
- 页码：68-75
- 文件大小：269 K
摘要
Stress-activated protein kinase-3 (SAPK3) is unique amongst the mitogen-activated protein kinase (MAPK) family with its C-terminal 5 amino acids directing interaction with the PDZ domain-containing substrates α1-Syntrophin and SAP90/PSD95. Here, we identify three additional PDZ domain-containing binding partners, Lin-7C, Scribble, and outer membrane protein 25 (OMP25). This latter protein is localised together with SAPK3 at the mitochondria but it is not a SAPK3 substrate. Instead, OMP25 inhibits SAPK3 activity towards PDZ domain-containing substrates such as α1-Syntrophin and substrates without PDZ domains such as the mitochondrial protein Sab. This is a new mechanism for the regulation of SAPK3 and suggests that its intracellular activity should not be solely assessed by its phosphorylation status.